silver staining host:plantpath.unl.edu

Listing 1 - 5 from 5 for silver staining

Les Lane's home page
... , My buffer page, - Crowle's stain Heat treatment for plant virus diagnosis (use with minipurification) Silver staining protocol for polyacrylamide gels (improved Morrissey) dsRNA isolation - useful variants of "Morris & Dodds" - protocol & overview ... Pathology 864B - course, Biochem 839 - course Plant Pathology 369L - lab, Foundations 101 Plant Path seminar silver stain, fluorescence in gels
plantpath.unl.edu

Gel electrophoresis tips and troubleshooting
... six months. Gel thickness - Minimizing gel thickness facilitates staining. With silver development, staining time increases with roughly the square of gel ... silver staining substantially! Advantages of silver staining - Silver development ("staining") is more sensitive than Coomassie Blue or ethidium bromide. It is quick and cheap (primary cost, as with Coomassie Blue, is organic solvents). Staining ...
plantpath.unl.edu

Crowle's stain
... no.1). Filter after each use. Staining procedure 1- Wash gel 2 hrs each with two changes of buffer (phosphate or Tris buffered saline). For silver staining, gels require more exhaustive washing. ... leads to high background with standard polyacrylamide staining procedures 8- Crocein scarlet is purely anionic and hence has little tendency for background staining 9- The basic procedure is from J. ...
plantpath.unl.edu

Improving minipurification
... .5 For SDS PAGE, make sure proteins are fully reduced (and alkylated if you're silver staining) Experiment with variables, but always use a series so that the trend is apparent Remember ...
plantpath.unl.edu

Preparing samples for SDS gel electrophoresis
... Preparing samples for SDS gel electrophoresis Home Silver stain Fluorescent staining Electrophoresis buffers Tips for SDS PAGE beginners Cracking buffer Preparing samples for SDS gel electrophoresis ... mercaptopropionic acid (bad odor) in the upper well. This causes "rain" if you stain with silver. Is the pH of your cracking buffer high enough for good reduction? Try several pHs ...
plantpath.unl.edu